{"product_id":"revvity-trf4004m","title":"Revvity, TRF4004M, LANCE Phospho-STAT3 (Tyr705 ) Detection Kit, 10,000 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nPlease note control lysates are NOT included in the kits. Control lysates are sold separately, catalog number TRF4004S.\u003cbr\u003e\nFormats:\u003c\/p\u003e\n\n\u003cp\u003eOur 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.\u003cbr\u003e\nOur 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.\u003c\/p\u003e\n\n\u003cp\u003eLANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665\/615 nm X 10,000).\u003cbr\u003e\nSTAT3 is an important transcriptional activator, and is involved in regulating cytokines and growth factor receptors. STAT3 is phosphorylated at Tyr-705 by the JAK pathway, upon which STAT3 will dimerize, translocate to the nucleus, and bind DNA. In some cancers STAT3 is shown to be constitutively activated. It has been implicated in both anti-apoptotic and oncogenic pathways. Application-Cell Signaling\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-LANCE Ultra\u003cbr\u003e\nCellular or Signaling Pathway-JAK\/STAT\u003cbr\u003e\nDetection Modality-LANCE\u003cbr\u003e\nMolecular Modification-Phosphorylation\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-15 µL\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTarget-STAT3\u003cbr\u003e\nTarget Class-Phosphoproteins\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Autoimmunity Neuroscience Respiratory\u003cbr\u003e\nUnit Size-10,000 Assay Points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775342727337,"sku":"TRF4004M","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-trf4004m","provider":"Iright","version":"1.0","type":"link"}