{"product_id":"revvity-trf4015c","title":"Revvity, TRF4015C, LANCE Phospho-EGFR (Tyr1068) Detection Kit, 500 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nPlease note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4015S.\u003cbr\u003e\nFormats:\u003c\/p\u003e\n\n\u003cp\u003eOur 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.\u003cbr\u003e\nOur 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.\u003c\/p\u003e\n\n\u003cp\u003eLANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665\/615 nm X 10,000).\u003cbr\u003e\nThe epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase for a number of extracellular ligands in the EGF family. Ligand binding triggers a number of events including dimerization and auto-phosphorylation. Several major signaling cascades are activated by EGFR. Application-Cell Signaling\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-LANCE Ultra\u003cbr\u003e\nDetection Modality-LANCE\u003cbr\u003e\nMolecular Modification-Phosphorylation\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-15 µL\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTarget-EGFR\u003cbr\u003e\nTarget Class-Phosphoproteins\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nUnit Size-500 Assay Points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775333945513,"sku":"TRF4015C","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-trf4015c","provider":"Iright","version":"1.0","type":"link"}