{"product_id":"revvity-trf4016m","title":"Revvity, TRF4016M, LANCE Total EGFR Detection Kit, 10,000 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nPlease note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4015S.\u003cbr\u003e\nFormats:\u003c\/p\u003e\n\n\u003cp\u003eOur 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.\u003cbr\u003e\nOur 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.\u003c\/p\u003e\n\n\u003cp\u003eLANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665\/615 nm X 10,000).\u003cbr\u003e\nThe epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase for a number of extracellular ligands in the EGF family. Ligand binding triggers a number of events including dimerization and auto-phosphorylation. Several major signaling cascades are activated by EGFR. Application-Kinase Protein Analysis \u0026amp; Detection\u003cbr\u003e\nAssay Points-10000\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-LANCE Ultra\u003cbr\u003e\nDetection Modality-TR-FRET\u003cbr\u003e\nTarget-EGFR\u003cbr\u003e\nTarget Class-Protein\u003cbr\u003e\nTherapeutic Area-Oncology\u003cbr\u003e\nUnit Size-10,000 Assay Points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775333847209,"sku":"TRF4016M","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-trf4016m","provider":"Iright","version":"1.0","type":"link"}