{"product_id":"revvity-trf4027m","title":"Revvity, TRF4027M, LANCE Phospho-AMPKα (Thr172) Detection Kit, 10,000 Assay Points","description":"\u003cp\u003eOverview\u003cbr\u003e\nPlease note control lysates are sold separately, catalog number TRF4027S.\u003cbr\u003e\nFormats:\u003c\/p\u003e\n\n\u003cp\u003eOur 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.\u003cbr\u003e\nOur 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.\u003c\/p\u003e\n\n\u003cp\u003eLANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665\/615 nm X 10,000).\u003cbr\u003e\nAMPKα is an isoform of AMP-activated protein kinase which plays a role in cellular energy homeostasis. Once bound by AMP or ADP, AMPK activations results in the stimulation of hepatic fatty acid oxidation, ketogenesis, and glucose uptake amongst other metabolic functions. Once the alpha subunit is phosphorylated at the T172 position, AMPK is considered activated. Application-Cell Signaling\u003cbr\u003e\nAutomation Compatible-Yes\u003cbr\u003e\nBrand-LANCE Ultra\u003cbr\u003e\nDetection Modality-LANCE\u003cbr\u003e\nMolecular Modification-Phosphorylation\u003cbr\u003e\nProduct Group-Kit\u003cbr\u003e\nSample Volume-15 µL\u003cbr\u003e\nShipping Conditions-Shipped in Blue Ice\u003cbr\u003e\nTarget-AMPKα\u003cbr\u003e\nTarget Class-Phosphoproteins\u003cbr\u003e\nTechnology-TR-FRET\u003cbr\u003e\nTherapeutic Area-Cardiovascular Neuroscience\u003cbr\u003e\nUnit Size-10,000 Assay Points\u003c\/p\u003e","brand":"Revvity","offers":[{"title":"Default Title","offer_id":46775341220009,"sku":"TRF4027M","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/revvity-trf4027m","provider":"Iright","version":"1.0","type":"link"}