{"product_id":"thermo-fisher-12605036","title":"Thermo Fisher, 12605036, TrypLE™ Express Enzyme (1X), phenol red","description":"\u003cp\u003eTrypLE™ Express Enzyme (1X), phenol red TrypLE Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A549, primary human keratinocytes, and embryonic stem cells. TrypLE Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts.\u003cbr\u003e\nWe offer a variety ofTrypLE formulationsfor a range of cell culture applications.\u003cbr\u003e\nCompared to trypsin and other dissociation reagents, Gibco TrypLE Express is:Gentle on cells\u003cbr\u003e\n•Room-temperature stable\u003cbr\u003e\n•Easy to use\u003cbr\u003e\n•Animal origin-free (AOF)This TrypLE Express is modified as follows:With\u003cbr\u003e\n• Phenol Red\u003cbr\u003e\n• EDTATheformulationof TrypLE solutions is available, although we keep the enzyme concentration confidential.\u003cbr\u003e\nGentle On CellsTrypLE Express' exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from enzymes present in some trypsin preparations and in other protease extracts.\u003cbr\u003e\nRoom-Temperature StableTrypLE Express is room-temperature stable and ready to use. TrypLE Express remains stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.\u003cbr\u003e\nEasy to UseTrypLE Express can be directly substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE Express, avoiding the need for trypsin inhibitors, such as FBS.\u003cbr\u003e\nAnimal Origin-Free (AOF)Unlike porcine trypsin, TrypLE Express is free of animal-derived components.\u003cbr\u003e\ncGMP Manufacturing and Quality SystemFor supply chain continuity, we manufacture Gibco TrypLE Express at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to the ISO 13485 standard, and are registered with the FDA as medical device manufacturers.\u003cbr\u003e\nCell Type: Mammalian Cells\u003cbr\u003e\nChelators: EDTA\u003cbr\u003e\nConcentration: 1 X\u003cbr\u003e\nCulture Type: Adherent Cell Culture\u003cbr\u003e\nGreen Features: Energy efficient, Sustainable packaging\u003cbr\u003e\nOsmolality: Check and Record\u003cbr\u003e\nProduct Line: Gibco™\u003cbr\u003e\nQuantity: 20 x 100mL\u003cbr\u003e\nRemarks: Room Temperature Stable\u003cbr\u003e\nShelf Life: 24 Months\u003cbr\u003e\nShipping Condition: Room Temperature\u003cbr\u003e\nSource: Animal origin-free\u003cbr\u003e\nTested For: In vitro Bioassay\u003cbr\u003e\nClassification: Animal Origin-free\u003cbr\u003e\nForm: Liquid\u003cbr\u003e\nProduct Type: Cell Culture Dissociation Reagent\u003cbr\u003e\nSterility: Sterile-filtered\u003cbr\u003e\nWith Additives: Phenol Red, EDTA\u003cbr\u003e\nUnit Size: Each\u003c\/p\u003e","brand":"Thermo Fisher","offers":[{"title":"Default Title","offer_id":45789570367657,"sku":"12605036","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/thermo-fisher-12605036","provider":"Iright","version":"1.0","type":"link"}