| Buffer | Running Buffer |
|---|---|
| Concentration | 10 X |
| For Use With (Application) | Nucleic Acid Gel Electrophoresis, Blotting |
| Gel Compatibility | Polyacrylamide Gels, Agarose Gels |
| Product Line | UltraPure |
| Product Type | TBE Buffer |
| Quantity | 1 L |
| Shipping Condition | Room Temperature |
| Format | Bottle |
| Unit Size | Each |
Answer:It contains 1 M Tris, 0.9 M boric acid, and 0.01 M EDTA.
Answer:It is suitable for both agarose and polyacrylamide gel electrophoresis.
Answer:The 1 L bottle is stored at room temperature.
UltraPure™ TBE Buffer, 10X is used to prepare 1X running buffer for agarose and polyacrylamide gel electrophoresis of nucleic acids. The sterile-filtered concentrate contains 1 M Tris, 0.9 M boric acid, and 0.01 M EDTA. Dilution to working strength provides the buffering capacity and ionic environment required for stable DNA or RNA migration during electrophoresis and blotting workflows. Quality testing detects no DNase, RNase, or protease activity, helping reduce the risk that the buffer itself will damage nucleic-acid samples. This configuration contains one 1 L bottle and is stored at room temperature. The ready-made 10X concentrate avoids repeated weighing and pH adjustment of individual chemicals and helps standardize gel-running conditions across experiments, especially in laboratories that routinely prepare large numbers of agarose or polyacrylamide gels.
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