Thermo Fisher, 26619, PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa, 2 x 250 μL

Name: PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa, 2 x 250 μL
Brand: Thermo Fisher
SKU: 26619

Quantity: 2 x 250 μL, Unit Size: Each

Catalog Number: 26619

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Category

Protein Ladders & Markers

Pack Size2 x 250 µL

TagsProtein Molecular Weight Markers Gel Electrophoresis Equipment and Supplies Gel Electrophoresis Reagents and Buffers

CollectionsThermo Fisher Gel Electrophoresis: Agarose Gels, SDS-PAGE & Blotting Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 2 x 250 μL
Unit Size: Each
Thermo Scientific PageRuler Plus Prestained Protein Ladder is a mixture of nine blue-, orange-, and green-stained proteins (10 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on SDS-polyacrylamide gels and western blots

Specifications

General

Detection Method	Colorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm)
Gel Compatibility	Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
Molecular Weight (g/mol)	250, 130, 100, 70, 55, 35, 25, 15, 10 kDa
Quantity	2 x 250 μL
Ready to Load	Yes
Shipping Condition	Approved for shipment on Wet or Dry Ice
Number of Markers	9
Product Line	PageRuler
Product Type	Protein Ladder
Size Range	10 to 250 kDa
Stain Type	Blue, Orange, Green
System Type	Western Blotting, SDS-PAGE
Unit Size	Each

FAQs

Question: What molecular-weight range does PageRuler Plus cover?

Answer:The ladder contains nine prestained proteins spanning approximately 10 to 250 kDa.

Question: Which bands serve as colored reference points?

Answer:Orange reference bands are located near 70 kDa and 25 kDa, and a green reference band is located near 10 kDa.

Question: Is sample preparation required before loading?

Answer:No. The ladder is supplied ready to use without heating, dilution or added reducing agent.

Application Note

PageRuler™ Plus Prestained Protein Ladder is used as a visible molecular-weight reference during SDS-PAGE and Western blotting. The ready-to-use ladder contains nine recombinant prestained proteins spanning approximately 10 to 250 kDa, allowing protein migration and transfer to be monitored across a broad molecular-weight range. Most bands are blue, while orange reference bands near 70 kDa and 25 kDa and a green reference band near 10 kDa provide easy visual orientation. No heating, dilution or additional reducing agent is required before loading. This configuration contains two 250 µL tubes and can be stored frozen for longer-term use. The colored reference bands are especially useful when checking electrophoresis progress, identifying molecular-weight regions, confirming membrane transfer and estimating the apparent size of target proteins.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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