Thermo Fisher, A2910001, ExpiCHO™ Expression Medium

Quantity: 1000 mL
Unit Size: Each
ExpiCHO Expression Medium is specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells. This medium is GMP-manufactured, chemically-defined, serum-free, protein-free, and animal origin–free. It is a core component of the ExpiCHO Expression System Kit (Cat. No. A29133) for scalable transient protein production. To achieve the highest levels of transient protein expression, the medium is matched to a culture feed, ExpiCHO Feed, also included in the system kit and transfection kits.With ExpiCHO Expression Medium you can:
• Grow suspension CHO culturesto densities of over 2 x 10^7cells per mL, with >95% cell viability
• Achieve high transfection efficienciesof >90% when coupled with ExpiFectamine CHO transfection reagent
• Transiently transfectsuspension CHO cells at a density of 6 x 10^6cells per mL
• Achieve yields up to 3 g per Lof recombinant protein of high-density transiently transfected cultures by sustained, high-level expression
• Grow and transfect CHO cellsat volumes of <1 mL up to 5 L in culture formats from multiwell plates up to 5 L shake flaskExpiCHO Expression Medium is ready to use. It is formulated with GlutaMAX-I reagent and does not require supplementation. It does not contain phenol red. The chemically-defined, animal origin free formulation results in high reproducibility and lot-to-lot reliability. ExpiCHO Expression Medium is not recommended for adherent cell cultures.