Thermo Fisher, F530L, Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Phusion™ High-Fidelity DNA Polymerase is a proofreading PCR enzyme designed for cloning and other amplification workflows where sequence accuracy is important. This colorless configuration is supplied at 2 U/µL and supports 500 reactions. The polymerase provides substantially higher fidelity than standard Taq DNA polymerase and supports short extension times of approximately 15–30 seconds per kilobase. The enzyme is engineered for robust amplification across a range of templates and can perform well in the presence of many common PCR inhibitors. Because this version uses a colorless buffer format, amplified products are not premixed with gel-loading dyes. The enzyme is suitable for high-fidelity PCR, cloning, mutagenesis, and preparation of templates for downstream sequence-sensitive applications.