Thermo Fisher, F530S, Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Phusion™ High-Fidelity DNA Polymerases (2 U/μL) is designed for accurate PCR amplification when sequence integrity and robust yield are important. This selected colorless version contains 100 units of Phusion DNA polymerase at 2 U/μL and is supplied for approximately 100 standard reactions. Its proofreading activity offers approximately 52-fold greater fidelity than conventional Taq DNA polymerase and generates predominantly blunt-ended products. The enzyme supports fast cycling with typical extension times of approximately 15–30 seconds per kilobase and amplification of fragments up to approximately 20 kb under appropriate reaction conditions. Applications include high-fidelity cloning, generation of sequencing templates, mutagenesis, difficult or GC-rich targets and other PCR experiments where incorporation errors should be minimized. The enzyme is a standalone reagent rather than a premixed 2X master mix, so reaction buffer, primers, template and other required components are assembled into the chosen PCR formulation. This product is the colorless version, rather than a green-buffer preparation intended for direct gel loading. The stated concentration and 100-reaction size should be retained when comparing it with the larger package options in the same family.