Thermo Fisher, Q32851, Qubit™ dsDNA Quantification Assay Kits

Name: Qubit™ dsDNA Quantification Assay Kits
Brand: Thermo Fisher
SKU: Q32851

Quantity: 100 assays, Assay: dsDNA Quantitation, HS, Quantitation Range: 0.1 to 120 ng, Unit Size: Each, Achieve accurate and precise quantification of dsDNA with Qubit dsDNA BR (Broad Range) Assay Kits. These dsDNA quantification kits enable quick and selective detection of low and high abundance DNA samples, and can distinguish dsDNA from ssDNA, RNA, protein, and free nucleotides. Contaminants such as salts, solvents, or detergents are well-tolerated.

Catalog Number: Q32851

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Category

Fluorescence Quantitation Kits

Pack Size100 assays

TagsMolecular Biology Reagents and Kits Nucleic Acid Labeling and Detection

CollectionsThermo Fisher Molecular Biology Reagents & Kits Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 100 assays
Assay: dsDNA Quantitation, HS
Quantitation Range: 0.1 to 120 ng
Unit Size: Each
Qubit dsDNA HS and BR Assay Kits, designed for use with Qubit Fluorometers, are highly selective for double-stranded DNA (dsDNA) over single-stranded DNA (ssDNA), RNA, protein, and free nucleotides. All kits provide concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 μL and 20 μL is acceptable), and read the concentration using a Qubit Fluorometer.Qubit dsDNA HS Assay KitThe Qubit dsDNA HS (High Sensitivity) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of sensitive DNA samples. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.005 to 120 ng/μL, providing a detection range of 0.1-120 ng.Qubit dsDNA BR Assay KitThe Qubit dsDNA BR (Broad-Range) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of DNA samples. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.2 to 2,000 ng/μL, providing a detection range of 4-2,000 ng.Notes
• Qubit dsDNA HS and BR Assay kits can be used with any Qubit Fluorometer
• Use with thin-wall, clear, 0.5-mL PCR tubes (Cat. No. Q32856) for the Qubit 4 Fluorometer and 8 x 200-μL tube strips (Cat. No. Q33252) for the Qubit Flex Fluorometer

Specifications

General

Assay	dsDNA Quantitation, HS
Excitation/Emission	510/527
For Use With (Equipment)	Qubit Fluorometer
No. of Reactions	100 Reactions
Product Line	Qubit
Quantitation Range	0.1 to 120 ng
Quantity	100 assays
Shipping Condition	Room Temperature
Detection Method	Fluorescence
Unit Size	Each

FAQs

Question: Is this the high-sensitivity or broad-range dsDNA assay?

Answer:This is the high-sensitivity dsDNA assay.

Question: What is the DNA quantitation range?

Answer:The assay measures 0.1–120 ng of double-stranded DNA.

Question: How many assays are supplied?

Answer:The kit contains reagents for 100 assays.

Application Note

Qubit™ dsDNA Quantification Assay Kits is designed for highly selective fluorescence quantitation of double-stranded DNA in samples containing low DNA amounts. This configuration is the high-sensitivity dsDNA assay and supplies reagents for 100 assays, with a DNA mass quantitation range of 0.1–120 ng. Its fluorescent chemistry preferentially detects double-stranded DNA over single-stranded DNA, RNA, protein, and free nucleotides, supporting more selective measurements than total nucleic acid absorbance. The supplied components include concentrated assay reagent, dilution buffer, and prediluted DNA standards. Prepare the working solution by diluting the reagent into its buffer, add a 1–20 µL sample aliquot, and measure fluorescence using a compatible Qubit Fluorometer. The supported starting-sample concentration range depends on the volume added and spans approximately 0.005–120 ng/µL. This assay is suited to checking low-abundance DNA prior to molecular biology workflows where input DNA concentration matters. It supports reproducible comparison of samples using the kit's prepared standards and an instrument-specific measurement workflow. The assay is intended for research use, not diagnostic procedures.

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