Thermo Fisher, S33102, SYBR™ Safe DNA Gel Stain

Name: SYBR™ Safe DNA Gel Stain
Brand: Thermo Fisher
SKU: S33102

Quantity: 400 μL, Unit Size: Each

Catalog Number: S33102

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Category

Nucleic Acid Stains

Pack Size400 µL

TagsGel Electrophoresis Equipment and Supplies Gel Electrophoresis Reagents and Buffers Electrophoresis Gel Stains

CollectionsThermo Fisher Gel Electrophoresis: Agarose Gels, SDS-PAGE & Blotting Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 400 μL
Unit Size: Each
SYBR Safe stain is specifically formulated to be a less hazardous alternative to ethidium bromide that can utilize either blue light or UV excitation.
• Reduces exposure to highly mutagenic ethidium bromide and harmful UV light
• Increases sensitivity by reducing nonspecific background fluorescence
• Use in place of ethidium bromide for all staining applications, including RNA staining
• Less hazardousA better DNA stain SYBR Safe DNA Gel Stain is a better nucleic acid staining reagent for molecular biology applications. Safer than ethidium bromide SYBR Safe DNA Gel Stain has been specifically formulated and evaluated in a battery of toxicity tests with results indicating that it is less mutagenic and safer than ethidium bromide. An independent laboratory showed reduced mutagenicity of SYBR Safe stain compared to ethidium bromide using the Ames test. Further decrease exposure risk by using visible blue-light illumination with SYBR Safe stain rather than UV illumination, especially when performing exposure-intensive protocols like cutting bands out of gels.Excellent sensitivity SYBR Safe stain offers excellent sensitivity in nucleic acid visualization and documentation applications with either UV excitation or blue-light excitation. When bound to nucleic acids, the green-fluorescent SYBR Safe stain has fluorescence excitation maxima at ∽280 and ∽502nm, and an emission maximum at ∽530nm. Plus, when used with blue light illumination, SYBR Safe stain has less background fluorescence than ethidium bromide–stained gels illuminated with UV light.Easy to use SYBR Safe stain is supplied as a 10,000X concentrate in DMSO which can be used like a solution of ethidium bromide. SYBR Safe stain can also be mixed into an agarose gel for staining during electrophoresis or the gel can be incubated in a solution of SYBR Safe stain following electrophoresis.Agarose Gel Electrophoresis, DNA and RNA Purification and Analysis, Nucleic Acid Gel Electrophoresis and Blotting

Specifications

General

Detection Location	In-Gel Detection
Detection Method	Fluorescence
Quantity	400 μL
Shelf Life	6 months from date of purchase
Shipping Condition	Room Temperature
Target Molecule	DNA
Label or Dye	SYBR Safe
Product Type	DNA Gel Stain
Unit Size	Each

FAQs

Question: What is SYBR Safe DNA Gel Stain used for?

Answer:It is used to visualize nucleic acids in agarose and polyacrylamide gels and can detect both DNA and RNA.

Question: What concentration is supplied?

Answer:The product is supplied as a 10,000X concentrate in DMSO.

Question: Can the stain be viewed with blue light?

Answer:Yes. It is compatible with blue-light excitation and can also be visualized using suitable UV illumination.

Question: What are the approximate fluorescence wavelengths?

Answer:The dye has excitation maxima near 280 and 502 nm and an emission maximum near 530 nm.

Question: How should the stain be stored?

Answer:Store at room temperature and protect from excessive light.

Application Note

SYBR™ Safe DNA Gel Stain is a fluorescent nucleic-acid stain designed for visualization of DNA in agarose or polyacrylamide gels and can also be used to detect RNA. The 10,000X concentrate is supplied in DMSO and can be incorporated into the gel or used according to supported post-staining procedures. The dye can be visualized with blue-light transilluminators and is also compatible with UV excitation, giving laboratories flexibility in gel-documentation workflows. Its fluorescence has excitation maxima near 280 and 502 nm and an emission maximum near 530 nm. The product is positioned as a safer alternative to ethidium bromide for routine nucleic-acid gel work. This configuration contains 400 µL; store at room temperature and protect from excessive light. It is for research use only.

Ordering

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