BD, 553689, BD Pharmingen™ Purified Rat Anti-Mouse CD86

Alternative Name: B7-2; Ly-58; Cd28l2; Early T-cell costimulatory molecule 1; ETC1; MB7; CLS1
Reactivity: Mouse (QC Testing)
Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
Immunogen: Mouse (CBA/Ca) LPS-activated splenic B Cells
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Blocking, Electron microscopy, Immunofluorescence, Immunoprecipitation (Reported)
Concentration: 0.5 mg/ml
RRID: AB_394991
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Mouse BD Fc Block™ purified anti-CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142) may help to reduce non-specific binding of GL1 antibody to cells bearing Fcγ-receptors. When performing immunofluorescent staining in the presence of Mouse BD Fc Block™, a second step antibody which does not cross-react with the 2.4G2 antibody (Rat IgG2b, κ) must be used. We have found that FITC-conjugated mouse anti-rat IgG2a (clone RG7/1.30, Cat. No. 553896) works well for this purpose. Immunohistochemistry : For IHC, we recommend the use of purified GL1 mAb in our special formulation for immunohistochemistry, Cat. No. 550542).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.