BD Pharmingen™ PE Mouse Anti-Mouse CD283 (TLR3) 565984

Alternative Name: Tlr3; TLR3; Toll-like receptor 3
Reactivity: Mouse (QC Testing)
Isotype: Mouse BALB/c IgG1, κ
Immunogen: Mouse TLR3 Transfected Cell Line
Application: Intracellular staining (flow cytometry) (Routinely Tested), Flow cytometry (Reported)
Concentration: 0.2 mg/ml
RRID: AB_2739436
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. An isotype control should be used at the same concentration as the antibody of interest.