Cytiva, RPN2134, Amersham™ ECL Western Blotting Detection Reagent

Amersham ECL Western Blotting Detection Reagent Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins. • Ideal for confirmatory Western blotting applications using chemiluminescence detection • At least 10-fold more sensitive than colorimetric assays • Detectable on Amersham Hyperfilm ECL or with a CCD imager such as ImageQuant LAS 500 . Discover our Western Blotting: tips and troubleshooting eLearning course . Learn approaches to ensure high-quality western blotting courses and receive a certificate upon course completion. High sensitivity chemiluminescence detection on Western blotting Amersham ECL Western Blotting Detection Reagents use enhanced luminol-based detection, which is suitable for all routine Western blotting. ECL Western Blotting Detection Reagents, in combination with Amersham Protran membranes, form a complete solution for Western blot applications.
Reliable chemiluminescence detection In most contemporary ECL systems a luminol peroxide detection reagent is added to the membrane and reacts with the horseradish peroxidase enzyme (HRP) conjugated to the secondary antibody. HRP catalyzes the oxidation of luminol in a multistep reaction and is accompanied by the emission of low-intensity light at 428 nm, which can be measured with light-sensitive X-ray film or with a CCD imager.