Waters, 186009427, Atlantis Premier BEH C18 AX Column, 5 µm, 4.6 x 50 mm, 1/pk

The Atlantis Premier BEH C18 AX Columns provide excellent retention for polar acidic analytes under reversed phase conditions. The reversed phase/anion-exchange mixed mode chemistry is stable from pH 2-10, and has complimentary selectivity to traditional C18 stationary phases, which is desirable when doing methods development.

Specifications
Chemistry: RP/Anion-exchange
Separation Mode: Mixed mode
Particle Substrate: Hybrid
pH Range Max: 10 pH
Temperature Limits: 60 C
Maximum Pressure: 18000 psi (1240 Bar)
Endcapped: Yes
Bonding Technology: C18 AX
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 5 µm
Endfitting Type: Parker-style
Pore Size: 95 Å
Format: Premier Column
Surface Area: 270
System: HPLC
Particle Technology: BEH
Technique: LC
USP Classification: L78
Inner Diameter: 4.6 mm
Length: 50 mm
Carbon Load: 17 %
UNSPSC: 41115709
Brand: Atlantis
Product Type: Columns
Units per Package: 1 pk

Additional Information
Atlantis Premier BEH C18 AX Column, 5 µm, 4.6 x 50 mm, 1/pk With a stable operating pH range between 2 and 10, the Atlantis Premier BEH C18 AX Column is a top pick for scientists who require supreme retention of polar acidic analytes in their liquid chromatography assays. This kind of uncompromising versatility is offered under reversed-phase/anion-exchange conditions. In addition, this HILIC silica column offers complimentary selectivity to traditional C18 stationary phases, which is something sought after by scientists everywhere. Enabling terrific separation performance is only a small part of the flexibility offered by the Waters Atlantis Premier column line. These HPLC columns can retain their sensitivity across multiple assays, which means you can reproduce results easily without any unwanted interactions. What’s more, the BEH C18 column chemistry is built with the high-retention BEH 95 Å particle, making the retention of polar acidic analytes easier than ever. The Atlantis Premier BEH C18 AX Column also has MaxPeak High Performance Surface technology built in to reduce surface and analyte interactions. This is especially helpful considering the polar acidic analytes being used for related LC assays. This Atlantis Premier HPLC column even has a charge modifier within it, helping to produce novel selectivity and increased retention of acids over a remarkably wide pH range. What makes this BEH C18 AX column more desirable than standard C18 column chemistries? For one thing, the ethylene-bridged hybrid (BEH) particle provides up to 50% more retention when compared to other C18 options. The hybrid particle, much like the charge modifier, allows for more flexibility in LC method development. Plus, this column is highly efficient and is designed to help reduce sample waste. Need a neutral sample while working with acidic compounds? Try the Neutrals QC Reference Material to ensure your chromatography systems are working with the proper benchmarks. This QC reference material will help you reduce waste and re-run time, while also being designed to be used with any chromatographic systems using a UV detector. At Waters, we offer a large catalog of mass spectrometry systems, lab automation products and other essential items you’ll need during your most critical assays. Shop for lab equipment at Waters to find all of the chromatography products required for a successful experiment with reliable results.

FAQ
How Do I Clean the Atlantis Premier BEH C18 AX Column? Although the cleaning solvent may vary depending on which compounds you’re working with, we recommend flushing your column with 20-column volumes of a neat, organic solvent before regeneration. Read more on BEH C18 AX column care in the user manual.