Cytiva, 17070901, DEAE Sepharose™ Fast Flow anion exchange chromatography resin

DEAE Sepharose Fast Flow, 500 mL DEAE Sepharose Fast Flow is a weak anion exchange chromatography resin (medium) for fast protein purification. • Well-proven weak anion exchanger developed for industrial downstream processes. • Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals. • High chemical stability allows for well proven CIP and sanitization protocols. • Hydrophilic base matrix ensures low levels of non-specific binding, and low levels of host cell-derived impurities in the elution pool. • Fulfills industrial demands for security of supply, robust performance, and regulatory support. Streamlined scale-up This resin is part of the Sepharose Fast Flow ion exchange platform, which is well established in industrial downstream processing. Composed of crosslinked 6% agarose beads with diethylaminoethyl (DEAE) weak anion exchange groups, the resin has high chemical stability, allowing well-proven cleaning-in-place (CIP) and sanitization protocols. The highly crosslinked agarose resin enables high flow rates and high productivity. Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, for example PreDictor 96-well plates, and PreDictor RoboColumns™, and HiTrap columns. Successful use – research scale DEAE Sepharose Fast Flow resin is used in a variety of research applications, including: Separation of PI 3-Kinases from guinea pig neutrophils Purification of myosin phosphatase from chicken smooth muscle Compatible empty columns – research scale Cytiva's solutions include a variety of empty columns for research scale. This DEAE resin is compatible with two of these lines, Tricorn and XK columns. Compatible empty columns – bioprocess scale For process-scale applications for this DEAE resin, Cytiva recommends AxiChrom columns. DEAE Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow. Prepacked columns – research scale This weak anion exchange resin is available in the following formats: HiScreen DEAE FF for scale up and process development HiPrep DEAE FF columns for convenient scale-up Prepacked columns – bioprocess scale Prepacked ReadyToProcess columns for bioprocess applications are available on request.