BD, 551459, BD Pharmingen™ Purified Rat Anti-Mouse Ly-6G

Reactivity: Mouse (QC Testing)
Isotype: Rat LEW, also known as Lewis IgG2a, κ
Immunogen: Ly-6G-transfected EL4J Cell Line
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-paraffin, Immunoprecipitation, Induction (Reported)
Concentration: 0.5 mg/ml
RRID: AB_394206
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Recommended Assay Procedure: For flow cytometry of leukocyte suspensions, we recommend the use of Mouse Fc Block™ (purified anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142). If Mouse Fc Block™ is used, it is important that the second-step antibody does not react with the 2.4G2 mAb (rat IgG2b, κ); we recommend FITC-conjugated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553896). Other reported applications include immunoprecipitation, immunohistochemical staining (IHC) of paraffin-embedded and acetone fixed frozen sections, and induction of T-cell inhibitory signalling. For IHC, we recommend the use of purified RB6-8C5 mAb in our special formulation for immunohistochemistry, Cat. No. 550291.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.