Thermo Fisher Immunoassay Reagents & Kits: ELISA, Multiplex, Plates & More

Thermo Fisher’s immunoassay portfolio helps you quantify protein biomarkers with confidence—from single-analyte ELISA kits to Luminex®-based multiplex panels, along with substrates, blockers, and high-binding plates that keep background low and signal high. On this Collection page, you can quickly compare formats and request the exact SKUs you need. As a distributor, Iright supplies a broad range of Thermo Fisher kits and reagents with technical support and reliable lead times.

What’s Inside: ELISA Kits, Multiplex Assays & Essential Reagents

You’ll find ready-to-run ELISA kits for classic cytokines and hormones, ProcartaPlex™ multiplex panels for one-well, many-analyte workflows, and the supporting chemistry—HRP substrates, blocking/wash buffers, and high-binding microplates—that determine assay consistency. The mix is designed to fit common plate readers and Luminex instruments so your lab can scale without retooling.

• ELISA kits (Invitrogen/eBioscience heritage): sandwich/competitive formats for cytokines (e.g., IL-6, TNF-α, IFN-γ), including phospho/total signaling kits (InstantOne™) for pathway studies.

• Multiplex immunoassays (ProcartaPlex™ on Luminex®): preconfigured 7- to 45-plex human cytokine/chemokine panels to maximize data from scarce samples.

• Detection reagents & buffers (Pierce™): chemiluminescent HRP substrates such as SuperSignal™ ELISA Pico, plus robust StartingBlock™ blockers for fast, uniform blocking in ELISA.

• High-binding plates (Nunc™ MaxiSorp™): certified, clear 96-well plates engineered for consistent antibody capture and low background.

Shop by Assay Type: ELISA, Luminex/ProcartaPlex, Substrates & Buffers

Selecting by methodology shortens your path to the right kit. Use this section to quickly access the product grid, which features filters for assay format, readout, platform, and sample type.

ELISA Kits (colorimetric/chemiluminescent): Choose single-analyte kits when you need maximum sensitivity, straightforward validation, and easy cross-study comparability. Serum, plasma, and cell-culture supernatants are commonly supported.

Multiplex Panels (ProcartaPlex™ on Luminex®): When sample volume is precious or you’re mapping immune networks, multiplex panels deliver 7–45 analytes in one well and are compatible with MAGPIX®, Luminex® 200, and FLEXMAP 3D. 

Detection Chemistry (HRP Substrates): Chemiluminescent substrates like SuperSignal™ ELISA Pico extend dynamic range and push limits of detection on luminometer-based readers.

Blocking/Wash Buffers: StartingBlock™ in PBS/TBS/TBST balances speed and low background across ELISA workflows. Pair with matched wash buffers for reproducibility.

Plates & Accessories: Nunc™ MaxiSorp™ plates (certified or non-certified) are a dependable foundation for consistent coating and low CVs.

Representative SKUs: Verified Thermo Fisher Catalog Numbers

To help you get started, here are frequently requested items across formats. These catalog numbers are verified on Thermo Fisher product pages; use them as references when you request quotes or datasheets from us.

Choosing the Right Format: Single-Analyte ELISA vs. Multiplex Panels

Your optimal path depends on study design, sample volume, and instrument access. Use ELISA for targeted quantitation with straightforward validation; switch to multiplex when you’re profiling networks or conserving precious plasma/serum.

• Single-analyte ELISA: Highest comparability across studies; ideal for primary endpoints or when regulatory documentation requires fixed assays.

• Multiplex (ProcartaPlex): Map cytokine signatures and discover co-regulated markers in one well; great for longitudinal cohorts with limited volume.

• Readout choice: Chemiluminescence (e.g., SuperSignal™) extends dynamic range and lowers LOD versus colorimetry—useful for low-abundance analytes.

• Signaling kits (InstantOne™): Quantify total and phospho-forms to track pathway activation without immunoblotting. 

• Plates & blockers matter: High-binding MaxiSorp™ plates plus optimized blocking (StartingBlock™) reduce background and improve CVs.

Applications & Target Families: Cytokines, Hormones, Oncology & More

Organize your shortlist by biological question. Most kits support serum, plasma, and cell-culture supernatants, with lysate-compatible options for phospho/total protein detection.

• Inflammation & Cytokines: IL-6, TNF-α, IFN-γ, and broader chemokine panels characterize immune status in infection, autoimmunity, and vaccine studies. Multiplex panels scale quickly when cohorts grow. 

• Endocrinology & Metabolism: Insulin and related hormones benefit from single-analyte ELISA for precision baselining across timepoints.

• Oncology & Immune Checkpoints: Use multi-marker panels to survey tumor-immune crosstalk; follow up with single-analyte ELISA for orthogonal confirmation.

• Signal Transduction: InstantOne™ kits quantify total/phospho pairs (e.g., ERK1/2) directly from lysates to capture pathway dynamics.

ELISA/Multiplex Workflow at a Glance: Coat–Incubate–Wash–Detect–Read

Whether you’re running a classic sandwich ELISA or a Luminex® assay, consistent technique drives data quality. Keep incubation timing, wash rigor, and plate handling uniform across plates and batches.

• Coat/Capture & Block: Use high-binding plates and a validated blocker to minimize nonspecific signal.

• Sample & Standard: Prepare curves and dilutions carefully; follow kit-specific volumes and matrices.

• Detect: Choose TMB or chemiluminescent HRP substrate appropriate for your instrument and desired LOD/dynamic range.

• Read & Analyze: Follow recommended wavelengths/timing (ELISA) or instrument settings (Luminex®), then apply curve fits consistent with the IFU.

FAQs: Sensitivity, Dynamic Range, Plate Type & Platform Compatibility

Q1: Do the Invitrogen ELISA kits work with serum and plasma?
Yes—most core cytokine kits (e.g., IL-6, TNF-α, IFN-γ) validate serum, plasma, and supernatants; check each IFU for volumes and ranges.

Q2: When should I use chemiluminescent detection?
Use chemiluminescent substrates (e.g., SuperSignal™ ELISA Pico) when you need lower LOD and a broader dynamic range than colorimetric TMB can provide.

Q3: Which plates are best for antibody coating?
Nunc™ MaxiSorp™ plates (certified options available) are a standard for high binding and lot-to-lot consistency in ELISA.

Q4: What multiplex sizes are available for human cytokines?
Common ProcartaPlex™ sets include 7-plex, 25-plex, 34-plex, and 45-plex panels; instrument compatibility includes MAGPIX®, 200, and FLEXMAP 3D.

Q5: Can I quantify phospho-proteins by ELISA instead of western blot?
Yes—InstantOne™ kits (e.g., ERK1/2 total/phospho) measure signaling states directly in lysates with plate readers.

Get a Quote or Talk to Iright’s Thermo Fisher Specialists

Tell us your target list, sample matrix, and instrument, and we’ll match the optimal kit or panel, then supply the verified SKU with IFU/datasheet. Iright can also coordinate cross-study lot matching and assist with plate/chemistry selection to stabilize your results over time.