BD, 553969, BD Pharmingen™ Purified Hamster IgG1, κ Isotype Control

Alternative Name: Anti-Trinitrophenol (TNP); TNP (Isotype Control Arm)
Isotype: Armenian Hamster IgG1, κ
Immunogen: TNP-keyhole limpet hemocyanin
Application: ELISA (Routinely Tested), Flow cytometry, Isotype control (Tested During Development)
Concentration: 0.5 mg/ml
RRID: AB_395169
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures An isotype control should be used at the same concentration as the antibody of interest (e.g., ≤ 1 µg/million cells for flow cytometry). For immunohistochemical staining, we recommend the use of purified A19-3 mAb in our special formulation for immunohistochemistry, Cat. No. 550344.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.