Thermo Fisher Chromatography Columns & Cartridges (HPLC/UHPLC, GC, IC, SPE)

Your chromatography work depends on consistent selectivity, rugged hardware, and formats that fit real-world methods. This collection brings together Thermo Scientific™ LC/UHPLC, GC, and ion chromatography (IC) columns alongside guard columns and HyperSep™ SPE cartridges—so you can build a complete, reproducible workflow from sample prep to final readout with fewer variables and easier reordering.

Product Overview: HPLC/UHPLC, GC & Ion Chromatography Columns, Guard & SPE

From routine QC to LC-MS trace analysis, each column family here targets a specific separation challenge. LC/UHPLC columns (e.g., Hypersil GOLD™, Accucore™) cover reversed-phase, PFP, HILIC, and normal phase. GC columns (TraceGOLD™) deliver low-bleed MS performance. IonPac™ IC columns resolve inorganic/organic ions across common eluents. Guard columns extend main-column life, while HyperSep SPE streamlines cleanup and enrichment.

In liquid chromatography, choose C18, PFP, or HILIC for small molecules and peptides, with particle sizes from sub-2 μm to 5 μm to balance speed and backpressure. For GC-MS, phase chemistry and film thickness control inertness, bleed, and sensitivity. IC columns pair capacity, selectivity, and eluent compatibility to your target anions or cations. Guard and SPE components stabilize retention and improve recovery in complex matrices.

Series at a Glance: Hypersil GOLD, Accucore, Syncronis, TraceGOLD, IonPac, HyperSep

Before diving into SKUs, a quick orientation helps you align series with outcomes. Think of these as “known-good defaults” that map cleanly to common methods and regulatory workflows.

• Hypersil GOLD™ (LC) – Fully porous, ultra-pure silica; excellent peak symmetry for LC-UV/LC-MS; multiple chemistries (C18, silica, etc.).

• Accucore™ (LC) – 2.6 μm solid-core particles for high efficiency at moderate backpressure; C18, PFP, Polar Premium options.

• Syncronis™ (LC) – Robust bonding and batch-to-batch reproducibility for routine methods.

• TraceGOLD™ (GC) – Low-bleed MS phases (e.g., TG-5MS) with outstanding inertness for semivolatiles, solvents, and pesticides.

• Dionex™ IonPac™ (IC) – Application-driven selectivity and capacity (e.g., AS19 for drinking-water anions).

• HyperSep™ (SPE) – Sorbents that handle polar and complex matrices (e.g., Hypercarb™, Retain PEP) prior to LC/GC.

How to Choose the Right Column or Cartridge (LC/GC/IC, SPE, Guard)

Start with the analyte and matrix, then match chemistry and dimensions to your detector and runtime constraints. Use the matrix below as a practical shortcut—pick the cell that matches your job, then fine-tune ID and length for throughput and sensitivity.

• Small molecules, LC-MS quant → C18 or PFP (Accucore/Hypersil GOLD); 2.1 × 100 mm; 1.9–2.6 µm for speed; consider guard if matrix is complex.

• Positional isomers / halogenated species → PFP (Accucore PFP); enhanced π–π/halogen selectivity; pair with volatile mobile phases.

• Peptides/polar metabolites → C18 with wider pores or HILIC; confirm MS compatibility; keep lines clean to protect guard columns.

• Semivolatiles/VOCs by GC-MS → TG-5MS with 0.25 µm film; length 30–60 m by resolution needs; inlet liners matter as much as the column.

• Drinking-water anions (IC) → IonPac AS19/AS19-4 µm; suppressor/eluent per method; ensure system backpressure margin.

• Dirty matrices (plasma/food/wastewater) → HyperSep SPE prior to LC/GC; choose reversed-phase vs mixed-mode by interferences; elute into mobile-phase-compatible solvent.

Featured SKUs: LC/GC/IC Columns & SPE Cartridges (High-Demand Specs)

Below are representative, frequently specified part numbers—verified against Thermo Fisher product pages—to anchor your internal lists and purchasing. Use them as starting points; the full assortment covers many more dimensions and chemistries.

Specifications & Compatibility: Particle/Pore, ID × Length, pH/Temp, LC-MS

Specs are more than numbers—they define peak shape, backpressure, and robustness. Use the short guide below to interpret trade-offs and avoid mismatches.

For LC, smaller particles (1.9–2.6 µm) increase efficiency and speed but raise pressure; 3–5 µm improve robustness on legacy HPLC. Narrower IDs (2.1 mm) increase sensitivity and reduce solvent use; 4.6 mm offers forgiving loading and backpressure. Pore sizes >100 Å help with peptides and larger analytes; standard 80 Å is ideal for most small molecules. Confirm pH/temperature windows if using aggressive conditions, and favor fully porous vs solid-core based on instrument limits and desired efficiency.

For GC, match phase polarity and film thickness to your analytes and matrix. Thin films (0.10–0.25 µm) favor speed and high-boiler resolution; thicker films (0.5–1 µm+) improve capacity for volatiles and dirty samples. Length (15–60 m) and ID (0.1–0.53 mm) balance speed, resolution, and carrier-gas constraints. TraceGOLD™ TG-5MS combines low bleed and inertness to protect MS sensitivity over longer sequences.

For IC, capacity and selectivity are built into each IonPac™ family; verify eluent strength and suppressor compatibility, plus backpressure on capillary vs standard formats. Wider-bore (4 mm) analytical columns suit routine methods; 2 mm or capillary sizes reduce eluent use and boost sensitivity when systems support them.

Applications & Methods: Pharma, Environmental, Food, Clinical, Petrochemical

Applications often map cleanly to a chemistry “lane.” Use the examples below to set defaults, then adjust dimensions for run-time or sensitivity.

• Pharma/Small-molecule QC – Hypersil GOLD™ C18 (or Accucore™ C18 for faster cycles) for related-substances and assay methods; keep ID at 2.1 mm for LC-MS. 

• Isomer-rich/halogenated compounds – Accucore™ PFP improves resolution for positional isomers and halogenated species, useful in stability studies and environmental screens.

• Semivolatiles/VOCs (GC-MS) – TraceGOLD™ TG-5MS phases are a proven baseline for solvent impurities, PAHs, pesticides, and phenols; choose 30–60 m lengths for complex lists.

• Drinking-water anions (IC) – IonPac™ AS19 or AS19-4 µm supports common EPA-style methods for fluoride, chloride, nitrate, and sulfate on carbonate/bicarbonate eluents.

• Difficult polar matrices – HyperSep™ Hypercarb™ or mixed-mode Retain PEP SPE removes interferences and concentrates analytes before LC-MS without diluting sensitivity.

Guard Columns & SPE Workflow: Protect Columns, Improve Recovery & Robustness

Guard columns are inexpensive insurance: match chemistry and (ideally) particle to the analytical column so the guard sacrificially traps particulates and late-eluting contaminants. SPE reduces matrix effects and extends column life; a consistent conditioning-load-wash-elute sequence improves reproducibility across operators.

Three quick playbooks

• Biofluids (plasma/serum): Protein-precipitate, then HyperSep SPE (e.g., reversed-phase or mixed-mode) to remove phospholipids; elute into MS-friendly solvent and protect the LC column with a short guard.

• Food extracts: Disperse fat-rich matrices; choose PFP/C18 for analytes, but SPE first to remove co-extractives that foul the GC inlet or LC column.

• Wastewater: Use SPE to pre-concentrate trace pollutants; for IC, prefilter particulates and validate suppressor capacity to maintain stable baselines.

FAQs: HPLC vs UHPLC, GC vs IC, Guard/SPE Pairing, LC-MS Considerations

This section captures the questions buyers and analysts ask most often so teams can move quickly from shortlists to POs without re-emailing methods teams.

1. How do I decide between HPLC and UHPLC formats?
Match particle size/ID to your instrument pressure limits. Sub-2 µm and 2.1 mm IDs need UHPLC-class pressure and tubing; 3–5 µm, 3.0–4.6 mm IDs run comfortably on legacy HPLC.

2. Do guard columns need to match the main column?
Yes—choose the same chemistry and similar particle size. Replace guards proactively to stabilize retention times and extend main-column lifetime.

3. Which GC film thickness is “default”?
For broad MS methods, 0.25 µm on a 30 m, 0.25 mm ID TG-5MS is a balanced starting point; go thicker for volatile-heavy or dirty samples. 

4. When do I add SPE?
Anytime matrix effects or low abundance threaten accuracy. Pick sorbent chemistry to bind interferences (or your analyte for elution), and elute with a mobile-phase-compatible solvent.

5. IC columns: does capacity affect sensitivity?
Capacity influences load tolerance and dynamic range; select a format (4 mm vs 2 mm/capillary) that fits eluent and detector sensitivity while keeping suppressor performance stable.

Request a Quote from Iright for Thermo Fisher Columns & Cartridges

Contact us to tell us your analyte, matrix, detector, and any method constraints. We’ll match Thermo Scientific™ columns and guards, add SPE where it meaningfully improves robustness, and share compatible alternatives so you can balance cost, lead time, and performance—then provide a fast quote and stocking options.