Thermo Fisher Laboratory Kits for qPCR, ELISA, Transfection & NGS

Thermo Fisher Laboratory Kits bring together ready-to-use reagents, controls, and protocols so you can move from sample to result with fewer variables and less setup time. This Collection page helps you compare use cases, instrument compatibility, and best-selling SKUs in one place. As a distributor, Iright provides a broad range of Thermo Fisher kits with fast quotes, dependable delivery, and technical guidance.

What Are Thermo Fisher Laboratory Kits?

Thermo Fisher kits bundle the core components required for a workflow—enzyme systems, optimized buffers, master mixes, and controls—together with step-by-step instructions. The goal is predictable performance and reproducibility across common lab tasks in molecular biology, protein analysis, immunoassays, and cell-based experiments.

You’ll typically find: a validated enzyme formulation (e.g., reverse transcriptase or hot-start Taq), a calibrated buffer/master mix (often 2X), a positive/negative control or standards for quantitation, and an Instructions for Use (IFU) that specifies reaction setup, cycling parameters, and recommended analysis steps. Kits are available for different sample types (blood, tissue, cultured cells, microbes), different throughputs (single tubes to 96/384-well plates), and common platforms (qPCR instruments, NGS, microplate readers, flow cytometers).

Applications & Kit Families: qPCR, ELISA, Transfection, NGS

Each application cluster below answers the core questions—what the kit does, when to choose it, and what it is compatible with—so you can navigate quickly to the right product family or data sheet without bouncing between pages.

Molecular Biology (cDNA, PCR/qPCR)
Use cDNA synthesis kits to convert RNA into first-strand cDNA for downstream qPCR (e.g., SuperScript IV VILO Master Mix for fast, sensitive RT in 10 minutes). Pair with SYBR Green or probe-based qPCR master mixes designed for tight Ct reproducibility and robust performance with GC-rich or low-input samples.

Protein & Antibody (Protein Quant, Western Support, Labeling)
For protein quantification before electrophoresis or Western blotting, BCA assay kits provide a broad linear range and compatibility with many detergents. Pierce™ BCA Protein Assay Kits include bovine serum albumin (BSA) standards and come in multiple sizes to match your throughput.

Immunoassays (ELISA & Multiplex)
ELISA kits for cytokines and biomarkers are available with pre-coated plates or Ready-SET-Go-style component kits, supporting single-plex workflows with calibrated standard curves. Choose ELISAs when you need absolute concentrations for QC, clinical research, or upstream screening.

Cell Biology & Transfection
When expression matters, lipid-based transfection kits like Lipofectamine™ 3000 provide efficient DNA/siRNA delivery across hard-to-transfect cells with streamlined protocols and consistent results at various scales (6-well through 96-well).

NGS Library Preparation
NGS kits such as Ion AmpliSeq™ Library Kit lines compress library construction into a short workflow with multiplex PCR panels, reducing hands-on time and batch variability while maintaining coverage uniformity. Options scale from 8 to 384 reactions to fit pilot runs or production cohorts.

How to Choose the Right Kit: Sample, Platform, RUO/IVD

Choosing well starts with your biological question and ends with your instrument and regulatory context. The path below keeps those constraints front-of-mind so you land on a kit that performs in your hands, on your platform, under your timeline.

Start with goal (quantify protein? measure gene expression? build NGS libraries?) → map to sample type (total RNA, cell lysate, plasma/serum, FFPE) → confirm detection method (SYBR vs probe, colorimetric vs chemiluminescence) → check instrument compatibility (qPCR system, plate reader, sequencer) → decide on controls & standards (included, optional, traceable) → confirm regulatory use (RUO or IVD) → align run size and turnaround.

Quick comparison (example fields to use on page):

Compatibility & Platforms: QuantStudio, Ion Torrent, Illumina

Instrument compatibility is one of the fastest ways to narrow options. Make sure your kit chemistry and cycling parameters align with your instrument’s optical channels, ramp rates, and software.

For qPCR, master mixes are broadly compatible with mainstream instruments, and many workflows call out proven use on Applied Biosystems™ QuantStudio™ systems. For NGS, AmpliSeq-based library prep targets Ion Torrent™ sequencers by design; Illumina®-compatible library prep kits are also available in other Thermo Fisher product families (e.g., Collibri). If you work across platforms, document dye/quencher compatibility, index sets, and read lengths in your lab notebook and purchasing notes.

Suggested on-page matrix fields: Platform → Supported Kit Families → Dye/Channel Notes → Plate/Tube Formats → Recommended Controls → Links to instrument pages.

Popular Kits & Best-Sellers: qPCR, ELISA, Lipofectamine, BCA

Featuring proven kits reduces decision friction and helps new users pick reliable starting points. Below are popular, widely adopted options with verified Thermo Fisher catalog numbers you can map to your PDPs and stock lists.

Quality, Compliance & Lot Consistency (RUO vs IVD)

Quality signals reassure buyers before they commit to a new workflow or scale up an assay. Your page should make validation and traceability visible—ideally near the kit name or price—so procurement doesn’t have to hunt for details elsewhere.

Summarize whether a kit is RUO (Research Use Only) or IVD, and link to Certificates of Analysis (CoA) or Conformance (CoC) where available. Call out lot-to-lot consistency metrics such as Ct variance for qPCR mixes, R² and slope acceptance for ELISA standard curves, or replicate CVs for protein assays. When relevant, mention storage conditions verified during stability testing (e.g., accelerated/real-time) and recommended freeze–thaw cycles. This information reduces post-purchase support tickets and makes audit prep easier.

Shipping & Storage: Cold-Chain, Stability, Shelf Life

Clear logistics language prevents delays and failed first runs. Some kits ship on dry ice (e.g., enzyme-heavy or master-mix products), while others are stable at 2–8 °C. State expected transit conditions, what to check upon arrival (intact cold packs, lack of thaw), and where to store components immediately.

Include shelf life and post-opening stability (e.g., months at 2–8 °C, recommended aliquoting to minimize freeze–thaw), plus brief guidance on handling sensitive reagents (protect from light, equilibrate to room temperature before setup). If you offer multiple shipping options, add a quick note about cut-off times and regional warehouses so buyers can plan their schedule.

Troubleshooting & FAQs: qPCR/ELISA/Transfection Issues

This section captures problem-intent searches and reduces back-and-forth with support. Keep answers concise and actionable, and point to IFUs or app notes for deeper dives.

Common issues & fixes (accordion-friendly bullets):

• qPCR: No amplification or late Cts. Check RNA integrity and remove inhibitors; verify primer design and annealing temperature; confirm master mix and instrument channel settings.
• qPCR: High variability between replicates. Mix master mix thoroughly, spin down plates, and avoid bubbles; calibrate pipettes; use a consistent plate seal.
• ELISA: Non-linear standard curve. Prepare fresh standards; ensure proper incubation times and temperatures; check plate washer carryover and substrate timing.
• ELISA: High background. Optimize blocking and washing steps; titrate detection antibody; verify plate reader wavelength settings.
• Transfection: Low efficiency. Confirm cell health and confluence; optimize DNA:reagent ratio; reduce serum during complex formation; consider cell-type-specific conditions (time, media change).
• NGS library prep: Low yield or biased coverage. Quantify input accurately; follow thermal cycling parameters precisely; verify panel compatibility and amplicon sizes.

When to contact support: Metrics drifting out of historical ranges, unexpected dye behavior on your qPCR system, repeated ELISA lot failures, or persistent transfection toxicity. Iright can help review run files, suggest kit alternatives, or coordinate with Thermo Fisher technical teams.