Agilent, 5065-4459, ZORBAX 300SB-C18, 3.5µm, 0.5 x 35 mm

ZORBAX 300SB-C18, 3.5µm, 0.5 x 35 mm. A reversed-phase C18 HPLC column with fully porous ZORBAX particles. StableBond technology ensures stability at low pH.

Specifications:
Brand: ZORBAX 300SB-C18
Hardware: SS
Inner Diameter (ID): 0.5 mm
LC Platform: Stainless Steel
Length: 35 mm
Maximum Temperature: 90 °C
Part Number Application Domain: Biopharma
Part Number Application: Monoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 3.5 µm
Particle Type: Fully Porous
Phase: 300SB-C18
Pore Size: 300 Å
Pressure Rating: 400 bar
Separation Mode: Reversed Phase
Shipping Solvent: Acetonitrile/Water
Technique: LCLC & LC/MS
UNSPSC Code: 41115709
pH Range: 1-8

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The Agilent ZORBAX 300SB-C18, 3.5µm, 0.5 x 35 mm column is engineered for high-resolution reversed-phase chromatography, specifically optimized for the separation of peptides and small proteins. Its stable, end-capped C18 bonded phase and 300 Å pore size are ideal for handling biomolecules with higher molecular weights, providing reliable performance in challenging analytical workflows. With a narrow internal diameter of 0.5 mm and a short 35 mm length, this column is particularly well suited for high-sensitivity LC-MS and nano- or capillary LC applications where sample volumes may be limited.

Researchers often employ this column for proteomics, biomarker discovery, and characterization studies requiring robust and reproducible separation of complex biological samples. Its advanced silica support ensures exceptional peak shape and minimal carryover, enhancing data integrity in high-throughput or regulatory environments. The column integrates seamlessly with Agilent 1100, 1200, and 1290 Infinity LC systems, as well as other nano- and capillary flow LC-MS platforms.

For optimal results, it is recommended to use compatible ZORBAX 300SB guard columns and Agilent-certified fittings. This column supports demanding workflows in pharmaceutical, clinical, and academic research laboratories where sensitivity and reproducibility are paramount.