Agilent, 5190-2413, Agilent Bio MAb, NP10, 4.6 x 250 mm

Agilent Bio MAb, NP10, 4.6 x 250 mm. This HPLC column has a densely packed weak cation exchange layer designed for monoclonal antibody separations. The particles are nonporous and promote higher resolution and faster separations.

Specifications:
Brand: Agilent Bio MAb
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 250 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: Charge Variant AnalysisPeptidesProteinsmAbs
Particle Size: 10 µm
Particle Type: Non-porous
Phase: WCX
Pressure Rating: 275 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-12

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Designed for high-resolution analysis of monoclonal antibodies and related biomolecules, this reversed-phase HPLC column features a nonporous NP10 bonded phase and measures 4.6 mm internal diameter by 250 mm length. Its robust silica substrate ensures exceptional separation efficiency, improving sensitivity and reproducibility in protein, antibody, and biopharmaceutical characterization workflows. The optimized particle surface minimizes secondary interactions and enhances recovery for intact proteins, fragments, and aggregates, facilitating detailed structural and purity assessments.

Ideal for applications in research and quality control laboratories, this column supports critical workflows such as monoclonal antibody mapping, protein isoform differentiation, and aggregate detection. It accommodates high-sensitivity detection demands, supporting analytical and preparative scale separations.

Compatible with a wide range of Agilent and third-party HPLC and UHPLC platforms, the column integrates smoothly with systems such as Agilent 1100, 1200, and 1260 Infinity Series LC units. For optimal performance, recommended mobile phases include aqueous-organic solvents with formic acid or trifluoroacetic acid modifiers. The column is also well suited for use alongside Agilent’s complete range of biopharmaceutical analysis consumables, enabling streamlined workflows from sample preparation through data interpretation.