Agilent, 5190-2473, Agilent Bio SAX, NP10, 4.6 x 250 mm

Agilent Bio SAX, NP10, 4.6 x 250 mm. A strong anion exchange HPLC column with nonporous particles. Particle size is 10 μm. Nonporous particles are best for efficient mass transfer and high resolution separations.

Specifications:
Brand: Agilent Bio SAX
Column Material: Stainless Steel
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 250 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: OligosProteins
Particle Size: 10 µm
Particle Type: Non-porous
Phase: SAX
Pressure Rating: 275 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-12

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The Agilent Bio SAX column, featuring NP10 resin and a 4.6 x 250 mm dimension, is designed for high-resolution strong anion exchange chromatography of biomolecules. Its optimized non-porous polymeric surface ensures enhanced binding capacity and superior peak shape, enabling precise separation of nucleic acids, proteins, monoclonal antibodies, and other charged biomolecules. Ideal for biopharmaceutical research, the column supports characterization and purification workflows such as charge variant analysis and protein isoform profiling. Offering robust chemical and mechanical stability, it delivers consistent performance across a wide pH range, compatible with both low and high ionic strength buffers for tailored method development. This product integrates seamlessly with popular Agilent LC systems, including the InfinityLab and 1290 series, and can be used alongside other Agilent Bio HPLC columns for comprehensive biomolecule analysis. Whether for routine quality control or advanced research applications, this strong anion exchange solution brings reliability and reproducibility to demanding laboratory environments. Its proven technology helps accelerate biomolecule discovery, process monitoring, and lot-to-lot consistency, meeting the requirements of regulated and research laboratory workflows.