Agilent, 5190-2529, Agilent Bio SEC-5, 300Å, 4.6 x 150 mm, 5 µm

Agilent Bio SEC-5, 300Å, 4.6 x 150 mm, 5 µm. A size exclusion column for aggregate analysis of peptides and small proteins. Resolving range 5-1,250 kDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 150 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 5000 Da
Molecular Weight Upper Limit: 1250000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 300 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

**********The following product description is generated by AI and is for reference only. If you have any questions, please contact customer service.**********
The Agilent Bio SEC-5, 300 Angstrom, 4.6 x 150 mm, 5 micron column is designed for high-resolution size exclusion chromatography of biomolecules. Engineered with a hydrophilic silica-based stationary phase, this column provides accurate molecular weight determination and separation of proteins, peptides, and other biological macromolecules. Its 300 Angstrom pore size ensures optimal performance when analyzing medium to large biomolecules, minimizing non-specific interactions and allowing for gentle sample handling.

Typical usage scenarios include protein aggregation studies, assessment of monoclonal antibody purity, and characterization of size variants in therapeutic proteins. It is also well suited for academic and industrial laboratories engaged in biopharmaceutical and life sciences research. The column is compatible with a range of HPLC and UHPLC systems, including Agilent 1100, 1200, and 1260 Infinity series, as well as other standard chromatography instruments.

For best performance, this column can be paired with Agilent’s full portfolio of HPLC-grade solvents and dedicated bio-inert accessories. Its robust design ensures reproducibility and longevity, making it a reliable choice for researchers seeking precise and efficient size-based biomolecule separation.