Agilent, 5190-2536, Agilent Bio SEC-5, 1000Å, 7.8 x 300 mm, 5 µm

Agilent Bio SEC-5, 1000Å, 7.8 x 300 mm, 5 µm. A size exclusion column for aggregate analysis of large biological molecules such as adeno associated virus and virus-like particles. Resolving range 15 kDa - 5 MDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 7.8 mm
LC Platform: Stainless Steel
Length: 300 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 50000 Da
Molecular Weight Upper Limit: 7500000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 1000 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The Agilent Bio SEC-5 column with 1000A pore size and dimensions of 7.8 x 300 mm, featuring 5 micron particle size, is specifically engineered for high-resolution size exclusion chromatography of biomolecules. This robust column provides excellent separation of proteins, monoclonal antibodies, and other biological macromolecules based on molecular size, enabling accurate molecular weight determination and aggregate analysis. Its stable silica-based packing with hydrophilic bonding minimizes nonspecific interactions, ensuring high recovery and reproducibility across multiple runs.

Ideal for biopharmaceutical development, quality control, and academic research, this column delivers reliable performance when separating analytes in complex biological samples or during protein purity assessments. The Bio SEC-5 series is compatible with most conventional HPLC and UHPLC systems, including Agilent 1100, 1200, and 1260 series, as well as other major liquid chromatography platforms. It seamlessly integrates with detectors such as UV, refractive index, or multi-angle light scattering for comprehensive analytical workflows. For extended application flexibility, other pore sizes and column dimensions are available within the Bio SEC-5 product family, supporting diverse needs from routine screening to advanced research and regulatory testing.