Agilent, 5190-2541, Agilent Bio SEC-5, 2000Å, 7.8 x 300 mm, 5 µm

Agilent Bio SEC-5, 2000Å, 7.8 x 300 mm, 5 µm. A size exclusion column for aggregate analysis of very large biological molecules. Resolving range 150 kDa to >10 MDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 7.8 mm
LC Platform: Stainless Steel
Length: 300 mm
Maximum Temperature: 80 °C
Molecular Weight Upper Limit: > 10,000,000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 2000 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The Agilent Bio SEC-5 column with a 2000 angstrom pore size and dimensions of 7.8 x 300 mm, featuring 5 micron particles, is designed for high-resolution size exclusion chromatography of biomolecules. It is particularly suited for the analysis of large proteins, antibodies, and aggregates in pharmaceutical and biopharmaceutical research. With its durable silica-based stationary phase and neutral surface chemistry, this column ensures accurate molecular size separation while minimizing non-specific adsorption and interactions.

The Bio SEC-5 column is commonly used in routine quality control, method development, and formulation studies for monitoring size variants including monomer and aggregate content in monoclonal antibodies and therapeutic proteins. It delivers reliable and reproducible results, supporting regulatory compliance for biologics characterization.

Compatible with most standard HPLC and UHPLC systems, this column integrates seamlessly with Agilent’s 1100, 1200, and 1260 Infinity LC series, as well as a wide range of third-party liquid chromatography platforms. The column can be paired with Agilent protein standards, guard columns, and mobile phases optimized for SEC workflows, providing a comprehensive solution for bioanalytical laboratories focused on protein characterization and stability studies.