Agilent, 5190-6854, Agilent Bio SEC-3, 100Å, 21.2 x 50 mm, 3 µm

Agilent Bio SEC-3, 100Å, 21.2 x 50 mm, 3 µm. A preparative scale size exclusion column for the purification of biological samples. Resolving range 0.1-100 kDa.

Specifications:
Brand: Agilent Bio SEC-3
Hardware: SS
Inner Diameter (ID): 21.2 mm
LC Platform: Preparative HPLC
Length: 50 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 100 Da
Molecular Weight Upper Limit: 100000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisPeptidesProteins
Particle Size: 3 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 100 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115709
pH Range: 2-8.5

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Engineered for precision size exclusion chromatography, this Agilent column features a pore size of 100 angstrom, dimensions of 21.2 by 50 millimeters, and a 3 micron particle size. It is designed to deliver high-resolution separation of proteins, peptides, and other biomolecules by molecular size, making it ideal for researchers conducting high-throughput bioanalysis, biomolecular characterization, or quality assessment of biotherapeutics. The column's advanced silica-based technology ensures exceptional efficiency, reproducibility, and minimal nonspecific binding, resulting in reliable quantitation and profiling of molecular size distributions.

This product is suitable for use in analytical, semi-preparative, and process-scale applications, accommodating a range of sample loads without sacrificing resolution. Typical research environments include pharmaceutical development, quality control laboratories, and academic institutions focusing on protein aggregation studies, antibody analysis, or biosimilar comparability assessments.

Compatible with Agilent InfinityLab LC systems and other major HPLC/UHPLC platforms, this column integrates seamlessly into existing workflows. It also pairs effectively with Agilent's range of Bio SEC-3 columns and matched guard columns, supporting full solution flexibility and extended column lifespan. Researchers benefit from robust performance across repeated runs, ensuring confidence in both routine and advanced applications.