Agilent, 5190-6866, Agilent Bio SEC-5, 500Å, 21.2 x 300 mm, 5 µm

Agilent Bio SEC-5, 500Å, 21.2 x 300 mm, 5 µm. A preparative scale size exclusion column for the purification of large biological samples such as adeno associated virus. Resolving range 15 kDa - 5 MDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 21.2 mm
LC Platform: Preparative HPLC
Length: 300 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 15000 Da
Molecular Weight Upper Limit: 5000000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 500 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115709
pH Range: 2-8.5

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The Agilent Bio SEC-5, 500 Angstrom, 21.2 x 300 mm, 5 micron column is engineered for high-performance size exclusion chromatography (SEC) of biological macromolecules. This robust column delivers excellent separation of proteins, peptides, and other biomolecules based on molecular size, making it highly suitable for applications in biopharmaceutical development, protein purity analysis, and aggregate evaluation. Its 500 Angstrom pore size and 5 micron particle design enable efficient resolution and reproducibility when analyzing samples such as monoclonal antibodies, enzymes, and complex protein mixtures.

Optimized for preparative and semi-preparative workflows, this column is compatible with a wide range of high-performance liquid chromatography HPLC and fast protein liquid chromatography FPLC systems, including Agilent and other major brands. It integrates seamlessly with related Bio SEC-5 columns of alternate dimensions, allowing method scalability and transferability. The durable silica-based particle construction supports excellent column lifetime and peak symmetry, even with complex samples.

Researchers rely on this column for confident molecular weight distribution analysis, oligomer detection, and monitoring of protein degradation products in both research and quality control environments. Its dependable performance makes it a versatile choice for laboratories focused on biomolecule characterization and process development.