Agilent, GX96-IAB, AdvanceBio Gly-X N-glycan prep with InstantAB kit, 96-ct

AdvanceBio Gly-X N-glycan prep with InstantAB kit, 96-ct. Complete kit utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid instant labeling of released N-glycans with InstantAB dye. With deglycosylation and labeling carried out in solution, the method is simple, rapid, and suitable for automation. After a simple cleanup step, the InstantAB-labeled glycan samples are ready for analysis by UHPLC, LC/MS, and other methods. Includes GX96-100 Gly-X deglycosylation module, GX96-501 Gly-X InstantAB labeling module, GX96-502 Gly-X InstantAB cleanup module.

Specifications:
Application: Flexible, high-throughput format:process 1 to 96 samples
Count: 96
Equipment & Reagents Provided by User: Thermocycler (Corning THERM-1001, 110V. THERM-1000, 230V). Note:Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating Blocks
Kit Configuration: Sample Prep Kit
Label: InstantAB
Prep time (approx): ~1 hour
Technique: LC

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The AdvanceBio Gly-X N-glycan prep with InstantAB kit, 96 count, offers an optimized solution for rapid, high-throughput N-glycan analysis from biotherapeutic glycoproteins. This comprehensive kit features streamlined sample preparation using an efficient Gly-X protocol, enabling enzymatic deglycosylation, clean-up, and fluorescent labeling with InstantAB dye in a single workflow. Users can process up to 96 samples in parallel, making it ideal for laboratories performing glycosylation profiling during biopharmaceutical development, biosimilar characterization, and quality control.

The InstantAB fluorophore provides excellent sensitivity and performance for downstream analysis such as UHPLC, HPLC, and LC-MS, allowing accurate quantification and robust structural elucidation of released N-glycans. The kit’s design supports automated and manual workflows and is fully compatible with Agilent InfinityLab LC systems, AdvanceBio Glycan Mapping columns, and associated data analysis tools. Typical users include researchers focused on monoclonal antibody characterization, vaccine development, and bioprocess monitoring. The platform aligns with regulatory requirements for glycan analysis, ensuring reliable and reproducible results. Agilent also provides a range of complementary products, including glycan standards and sample clean-up solutions, to further streamline and support comprehensive glycan analytical workflows.