BD, 550431, BD Pharmingen™ Biotin Mouse Anti-Human CD253

Alternative Name: TNFSF10; TRAIL; APO-2L; TL2
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1
Immunogen: Human TRAIL Transfected Cell Line
Application: Flow cytometry (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_393679
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent Staining and Flow Cytometry : Applications include flow cytometry (0.25-1.0 µg/1x10e6 cells). Blocking : The RIK-2 antibody is useful to block TRAIL-induced apoptosis. The no azide/low endotoxin format (NA/LE), Cat. No. 550912,  is recommended for in vitro blocking assays.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.