BD, 551130, BD Pharmingen™ Recombinant Human IP-10

Preparation And Storage: Preparation And Storage This preparation contains no preservatives, thus it should be handled under aseptic conditions. Store product at -80°C prior to use or for long term storage of stock solutions. Avoid multiple freeze-thaws of product. Recombinant human IP-10 is supplied lyophilized from a solution of aqueous buffered solution containing 50 µg BSA per 1 µg of cytokine as carrier protein. No sodium azide has been added to the preparation and the endotoxin level is less than 1 EU per 1 µg of the cytokine as determined by the LAL method. The product can be reconstituted in sterile neutral buffer containing not less than 0.5 - 10.0 mg/ml carrier protein such as human or bovine serum albumin, aliquoted into polypropylene microtubes and stored at -80°C. NOTE: Failure to add carrier protein** or store at indicated temperatures may result in a loss of activity.
Recommended Assay Procedures: Recommended Assay Procedures ELISA Standard: The recombinant human IP-10 (Cat. No. 551130) is useful as a quantitative standard for a sandwich ELISA for measuring human IP-10 protein levels. Purified 4D5 antibody (Cat. No. 555046) can be paired with the biotinylated 6D4 antibody (Cat. No. 555048) with recombinant human IP-10 as the standard. To obtain linear standard curves, doubling dilutions of human IP-10 ranging from ~1,000 to 5 pg/ml are  recommended for inclusion in each ELISA plate. For specific methodology please visit the protocols sections or the chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com. Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring human-IP-10 in serum or plasma our BD OptEIA™ Human IP-10 Set (Cat. No. 550926) is specially formulated and recommended. Note: Biological Activity: This preparation of recombinant human IP-10 protein has not been evaluated for biological activity. ** Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity .