BD, 551249, BD Pharmingen™ Purified Mouse Anti-Human CD209

Alternative Name: DC-SIGN
Reactivity: Human (QC Testing)
Isotype: Mouse IgG2b, κ
Immunogen: Human Monocyte Derived DC Cells
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-paraffin (Not Recommended)
Concentration: 31.25 µg/ml
RRID: AB_394120
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: This antibody is recommended to test for immunohistochemical staining on acetone-fixed frozen sections from either human spleen or tonsil and reported to detect dendritic cells. IHC of formalin-fixed paraffin embedded sections is not recommended . For optimal indirect immunohistochemical staining, the DCN46 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with biotinylated polyclonal anti-mouse Ig (Cat. No. 550337) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). More conveniently, the anti-mouse Ig HRP detection kit (Cat. No. 551011) can be used which contains the biotinylated secondary antibody, antibody diluent, streptavidin-HRP and a DAB substrate for use in the staining procedure.