BD, 551320, BD Pharmingen™ Purified Rat Anti-Mouse Follicular Dendritic Cell

Reactivity: Mouse (QC Testing)
Isotype: Rat IgG2c, κ
Application: Immunohistochemistry-frozen (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
RRID: AB_394151
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The FDC-M1 clone reactive against mouse follicular dendritic cells is tested for immunohistochemical staining of acetone-fixed frozen sections. Tissues tested were mouse spleen, thymus and small intestine. The antibody stains follicular dendritic cells and their processes. For optimal indirect immunohistochemical staining, the FDC-M1 antibody should be titrated (1-10 to 1-50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-rat Igs (multiple adsorbed) (Cat. No. 559286) as the secondary antibody and  treptravidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). More conveniently, the anti-rat Ig HRP detection kit (Cat. No. 551013) that provides the biotinylated secondary antibody, antibody diluent and the detection system can be used to stain for anti-mouse follicular dendritic cells. Adetailed protocol of the immunohistochemical procedure is enclosed. The clone FDC-M1 is not recommended for formalin-fixed paraffin embedded sections.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States.