BD, 551850, BD Pharmingen™ PE Mouse Anti-Human CD126

Alternative Name: Interleukin 6 Receptor alpha chain; IL-6R alpha; IL-6Rα
Reactivity: Human (QC Testing)
Isotype: Mouse BALB/c IgG1, κ
Immunogen: CD126 Recombinant Protein
Application: Flow cytometry (Routinely Tested)
Vol. Per Test: 20 µl
Workshop Number: VI C63; IX 36
RRID: AB_394271
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent Staining and Flow Cytometric Analysis: The PE Mouse Anti-Human CD126 (Cat. No. 551850) can be used for the immunofluorescent staining (20 µg/10e6 cells) and flow cytometric analysis of human nucleated cells to measure their expressed levels of surface IL-6Ra.15 An appropriate purified immunoglobulin is PE Mouse IgG1, κ Isotype Control (Cat. No. 555749). ELISA: Purified Mouse Anti-Human CD126 (Cat. No. 551462) is useful as a capture for a sandwich ELISA that measures soluble human IL-6Ra protein levels. The M5 antibody can be paired with the M182 antibody and recombinant soluble human IL-6Ra as a standard.
Product Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Ficoll-Paque is a trademark of Amersham Biosciences Limited. Source of all serum proteins is from USDA inspected abattoirs located in the United States. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.