BD, 553693, BD Pharmingen™ Purified NA/LE Rat Anti-Mouse CD121a

Alternative Name: Il1r1; IL-1 Receptor Type I/p80; IL-1 R alpha; IL-1 Rα; IL-1 RI
Reactivity: Mouse (QC Testing)
Isotype: Rat IgG1, κ
Immunogen: IL-1 receptors from the C57BL/6 mouse T lymphoma EL4.IL2
Application: Flow cytometry (Routinely Tested), Blocking, Immunoprecipitation, Western blot (Reported)
Concentration: 1.0 mg/ml
RRID: AB_398537
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Recommended Assay Procedures: Recommended Assay Procedures Flow cytometry: Since this antigen may be expressed at low density on the cell surface, investigators may wish to perform staining using Biotin Goat Anti-Rat Ig (Cat. No. 554014) coupled with a "bright" third-step reagent, such as PE Streptavidin (Cat. No. 554061). Western Blot: The 35F5 antibody has been reported to be useful for western blotting of non-reduced proteins and being non-reactive to reduced proteins.