BD, 554007, BD Pharmingen™ Biotin Mouse Anti-Armenian and Syrian Hamster IgG1

Reactivity: Hamster (QC Testing)
Isotype: Mouse BALB/c IgG2b, κ
Immunogen: Pooled Armenian hamster IgG mAb
Application: ELISA (Routinely Tested), Flow cytometry (Tested During Development)
Concentration: 0.5 mg/ml
RRID: AB_395203
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures For the sandwich hamster IgG (group 1) ELISA, biotin-conjugated mAb G94-56 is optimal for detection with purified anti-hamster IgG mAb HIG-632 (Cat. No. 550637) for capture and Cat. No. 553969 as protein standard. Biotin-conjugated G94-56 mAb may be used as a secondary reagent in immunofluorescent staining. For immunohistochemical staining, we recommend the use of biotinylated anti-hamster IgG mAb cocktail in our special formulation for immunohistochemistry, Cat. No. 550335.