BD, 554130, BD Pharmingen™ Purified Mouse Anti-MyoD

Reactivity: Human (QC Testing), Mouse, Rat, Chicken (Tested in Development)
Isotype: Mouse IgG1, κ
Immunogen: Recombinant Mouse MyoD
Application: Western blot (Routinely Tested), Immunohistochemistry-frozen (Tested During Development)
Target Molecular Weight: 45/48 kDa
Concentration: 0.5 mg/ml
RRID: AB_395255
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Applications include Western blot analysis (1-2 µg/ml) and immunohistochemical staining of paraformaldehyde-fixed, frozen tissue sections and myogenic cell lines (titrate between 0.5-5 µg/ml).  RD Rhabdomysarcoma cells (ATCC CCL-136) and normal myoblasts are suggested as a positive control for these applications. Cell lines which are suggested as negative controls include: Ewing's sarcoma cells RD-ES (ATCC HBT-166), neuroblastoma cells SK-N-SH (ATCC HTB-11), and Burkitts lymphoma cells EB2 (ATCC HTB-61).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application. Please refer to http://regdocs.bd.com to access safety data sheets (SDS). Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.