BD, 554149, BD Pharmingen™ Purified Mouse Anti-SV40 Large T Antigen

Reactivity: Viral (QC Testing)
Isotype: Mouse IgG2a
Immunogen: SV40-transformed BALB/c mouse cell lines
Application: Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry-formalin (antigen retrieval required), Immunoprecipitation (Reported)
Target Molecular Weight: 85-100 kDa
Concentration: 0.5 mg/ml
RRID: AB_395269
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures T-ag purified with PAb 101 has been shown to retain helicase activity. T-ag is immunoprecipitated as a single or multiple bands between about 80-95 kD depending on post-translational modifications. SV40-transformed cells such as COS-7 (ATCC CRL 1651) are suggested as positive controls. Any cell line that is not SV40-transformed or SV40-infected can be used as a negative control.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.