BD, 554257, BD Pharmingen™ FITC Hamster Anti-Mouse CD95

Alternative Name: Apo-1; Apt1; Fas; FASLG receptor; lpr; TNFR6; Tnfrsf6; TNR6
Reactivity: Mouse (QC Testing)
Isotype: Armenian Hamster IgG2, λ2
Immunogen: WR19L mouse lymphoma cells transformed with recombinant mouse Fas
Application: Flow cytometry (Routinely Tested)
Target Molecular Weight: 45 kDa
Concentration: 0.5 mg/ml
RRID: AB_395329
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Recommended Assay Procedures: Recommended Assay Procedures The NA/LE format (No Azide/Low Endotoxin, Cat. No. 554254) of Jo2 should be used for both in vitro and in vivo apoptosis assays. The other formats contain azide and have not been specifically prepared with low endotoxin levels. The presence of sodium azide and/or endotoxin in other formats may affect the results of functioal assays, both in vitro and in vivo.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.