BD, 554466, BD Pharmingen™ FITC Rat Anti-Mouse IL-10

Alternative Name: Interleukin-10; Il10; CSIF; Cytokine synthesis inhibitory factor
Reactivity: Mouse (QC Testing)
Isotype: Rat IgG2b
Immunogen: Recombinant mouse IL-10
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_395411
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent Staining for Flow Cytometric Analysis : The JES5-16E3 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify and enumerate IL-10 producing cells within mixes cell populations. The FITC-conjugated JES516E3 antibody (Cat. No. 554466;) is especially suitable for these experiments (see figure, left panel). For optimal immunofluorescent staining with flow cytometric analysis, this anti-cytokine antibody should be titrated (≤ 0.5 µg mAb/ million cells). The use of a specificity control, such as one of the following, is suggested: 1) recombinant mouse IL-10 (Cat. No. 550070) unlabeled JES5-16E3 antibody, (Cat. No. 554464), and 3) rat IgG2b isotype control, FITC-A95-1 (Cat. No. 556923). Isotype control should be used at comparable concentration to antibody of interest. For specific methodology, visit the protocols section of the website, or see the Techniques for Immune Function Analysis Application Handbook, Chapter 4: Immunofluorescent Staining of Intracellular Molecules for Flow Cytometric Analysis , both of which are located at www.bdbiosciences.com. Neutralization: The NA/LE™ FES5-16E3 antibody (Cat. No. 554463) is useful for neutralization of mouse IL-10 bioactivity. ELISA Detection: The biotinylated JES5-16E3 antibody (Cat. No. 554465) is useful as a detection antibody for a sandwich ELISA for measuring mouse IL-10 protein levels. For testing IL-10 in serum or plasma, our OptEIA™ Set (Cat. No. 555252) is recommended.