BD, 554717, BD Pharmingen™ Purified Mouse Anti-Human IL-8

Reactivity: Human (QC Testing)
Isotype: Mouse IgG2b
Immunogen: Recombinant Human IL-8
Application: Intracellular block/flow cytometry (Routinely Tested), ELISA, Flow cytometry (Tested During Development)
Concentration: 0.5 mg/ml
RRID: AB_398583
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Blocking Control for Intracellular Staining: The purified G265-8 antibody (Cat. No. 554717) can be used as a blocking control to demonstrate specificity of IL-8 staining by PE-G265-8 antibody (Cat. No. 554720). To perform this control, the fixed/permeabilized cells (~ 1 million) can be incubated with 1-10 µg of unlabeled G265-8 antibody (Cat. No. 554717) for 20 minutes at 4°C, prior to staining with PE-G265-8 antibody (e.g., 0.1 - 0.5 µg mAb/1 million cells). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe. ELISA Detection: The biotinylated G265-8 antibody (Cat. No. 554718) is useful as a detection antibody for a sandwich ELISA for measuring human IL-8 protein levels. Biotinylated G265-8 antibody can be paired with the purified G265-5 antibody (Cat. No. 554716) with recombinant human IL-8 (Cat. No. 554609) as the standard. For testing IL-8 in serum or plasma, our human IL-8 OptEIA™ ELISA Set (Cat. No. 555244) and Kit (Cat. No. 550999) are specially formulated and recommended. Immunofluorescent Staining and Flow Cytometric Analysis: The G265-8 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify and enumerate human IL-8 producing cells within mixed cell populations. The PE-conjugated G265-8 antibody (Cat. No. 554720) is especially suitable for these experiments. For specific methodology, please visit the protocols section or chapter on intracellular staining in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com. Immunocytochemical staining : The purified format of G265-8 antibody is useful for ICC staining with a DAB substrate. The Cat. No. 550419 is tested in the ICC application.