BD, 555049, BD Pharmingen™ PE Mouse Anti-Human IP-10

Reactivity: Human (QC Testing)
Isotype: Mouse IgG2a, κ
Immunogen: Recombinant human IP-10 protein
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_395670
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent Staining and Flow Cytometric Analysis: The PE-conjugated 6D4/D6/G2 antibody (Cat. No. 555049) can be used for multicolor immunofluorescent staining and flow cytometric analyses to identify and enumerate human IP-10-producing cells within mixed cell populations (See Figure).  For optimal immunofluorescent staining with flow cytometric analysis, this anti-cytokine antibody should be titrated.  (≤ 0.5 µg mAb/1X10^6 cells) For specific methodology, please see the online protocols or the chapter on intracellular staining in the Immune Function handbook posted at www.bdbiosciences.com. A useful control for demonstrating specificity of staining is to pre-block the paraformaldehyde-fixed/saponin-permeabilized cells with unlabeled 6D4/D6/G2 antibody prior to staining. The staining technique and use of blocking controls are described by C. Prussin, et al. A suitable mouse IgG2a isotype control for assessing the level of background staining on paraformaldehyde-fixed/saponin-permeabilized human cells is PE-G155-178 (Cat. No. 554648); use at comparable concentrations to antibody of interest (e.g., ≤ 0.5 µg mAb/1X10^6 cells). ELISA: The biotinylated 6D4/D6/G2 (Cat. No. 555048) antibody is useful as a detection antibody for a sandwich ELISA for specifically measuring human IP-10 protein levels. Biotin 6D4/D6/G2 antibody can be paired with the Purified 4D5/A7/C5 antibody (Cat. No. 555046) as the capture antibody and with Recombinant human IP-10 protein (Cat. No. 551130) as the standard.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. An isotype control should be used at the same concentration as the antibody of interest. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.