BD, 555106, BD Pharmingen™ Recombinant Rat IL-2

Preparation And Storage: Preparation And Storage This preparation contains no preservatives, thus it should be handled under aseptic conditions. Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at –80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein, such as human or bovine albumin, aliquoted and stored at ≤ -70°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 – 1.0 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. The product should not be diluted to less than 10 μg/ml for long term storage. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.
Recommended Assay Procedures: Recommended Assay Procedures 1. Biological Activity Activity is tested: in a Proliferation assay using CTLL-2 indicator cells Specific activity : 0.6 - 5.0 x 10^8 Units*/mg ED50 : 20 - 150 pg/ml Observed Dose response : 100 fold * A Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation. Note : This application is not routinely tested at BD Biosciences. 2. ELISA Standard Recombinant rat IL-2 is useful as a quantitative standard for measuring rat IL-2 protein levels in an IL-2 specific sandwich ELISA. To obtain linear standard curves, doubling dilutions of this rat IL-2 standard from ~2,000 to 15 pg/ml should be included in each ELISA plate.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Please refer to http://regdocs.bd.com to access safety data sheets (SDS). Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.