BD, 556370, BD Pharmingen™ Purified Mouse Anti-Human CD95

Alternative Name: Fas/APO-1
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1
Immunogen: Recombinant Human Fas
Application: Western blot (Routinely Tested), ELISA Capture (Tested During Development), Flow cytometry, Functional assay (Not Recommended)
Target Molecular Weight: 45 kDa
Concentration: 0.5 mg/ml
RRID: AB_396391
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Applications include western blot analysis (1-2µg/ml). Clone G254-274 has also been tested in antibody development for ELISA where G254-274 has been used as a capture antibody paired with the biotin mouse anti-human Fas clone DX2 (MN 555672) as the detection antibody. The BD OptEIA™ Set for soluble, human Fas (MN 555224) is the suggested format for use in ELISA analysis. Clone G254-274 is not suggested for flow cytometric analysis or for use in functional apoptosis assays.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.