BD, 556439, BD Pharmingen™ Recombinant Human p53 Protein (Wildtype)

Preparation And Storage: Preparation And Storage This preparation contains no preservatives, thus it should be handled under aseptic conditions. Avoid multiple freeze-thaws of product. The protein solution was sterile filtered and frozen at -20°C. Store the protein at -20°C; it may also be stored at -80°C.
Recommended Assay Procedures: Recommended Assay Procedures 1 µg of protein can easily be visualized on a Coomassie Blue stained SDS/PAGE as a band of 53 kDa. For Western blot analysis, 50 ng or less is sufficient for the chemiluminescence detection method. The p53 recombinant protein has not been evaluated for suitability in other assays outside of Western blot analysis.