BD, 557192, BD Pharmingen™ APC Rat Anti-Mouse CD25

Alternative Name: Interleukin-2 receptor alpha chain; IL-2RA; IL-2Rα; Il2ra; IL-2R p55
Reactivity: Mouse (QC Testing)
Isotype: Rat OFA, also known as Outbred OFA IgG1, λ
Immunogen: IL-2-dependent cytolytic mouse T-cell clone B6.1
Application: Flow cytometry (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_398623
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
Recommended Assay Procedures: Recommended Assay Procedures The APC fluorochrome is excited by laser lines from 595 to 647 nm, and its emission is collected in a detector for fluorescence wavelengths between 640 and 680 nm. For detection of low-density CD25 expression, we recommend the use of the PE conjugate of PC61 antibody (Cat. No. 553866) or the biotin conjugate of the 7D4 antibody (Cat. No. 553069/553070) with a "bright" second-step reagent, such as Streptavidin-PE (Cat. No. 554061). Since applications vary, each investigator must determine dilutions appropriate for individual use.