BD, 557539, BD Pharmingen™ Purified NA/LE Hamster Anti-Mouse IL-1β

Reactivity: Mouse (QC Testing)
Isotype: Armenian Hamster IgG, λ1
Immunogen: Mouse IL-1β Protein
Application: ELISA (Routinely Tested), Neutralization (Tested During Development), Immunoprecipitation (Reported)
Concentration: 1.0 mg/ml
RRID: AB_396752
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Recommended Assay Procedures: Recommended Assay Procedures ELISA : Purified Hamster Anti-Mouse IL-1β (Cat. No. 550605) has been reported to be useful as a capture antibody for sandwich ELISA measuring mouse IL-1 β protein levels.   For measuring mouse IL-1β in complex biological samples, such as serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Mouse IL-1β ELISA Set (Cat. No 559603). Immunoprecipitation :  Purified Hamster Anti-Mouse IL-1β (clone B122) has been reported to be useful for the immunoprecipitation of both natural and recombinant forms of mouse IL-1 β protein.  Please note that this antibody is not routinely tested for this application.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.