BD, 557649, BD Pharmingen™ PE-Cy™7 Rat Anti-Mouse IFN-γ

Reactivity: Mouse (QC Testing)
Isotype: Rat IgG1, κ
Immunogen: Mouse IFN-γ Recombinant Protein
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_396766
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PE-Cy7 under optimum conditions, and unconjugated antibody and free PE-Cy7 were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent Staining and Flow Cytometric Analysis: The PE-Cy-7 conjugated XMG1.2 antibody is useful for the immunofluorescent staining and flow cytometric analysis to identify and enumerate IFN-γ producing cells within mixed cell populations. For optimal immunofluorescent staining with flow cytometric analysis, this anti-cytokine antibody should be titrated (≤ 0.125 µg mAb/million cells).  A useful control for demonstrating specificity of staining is to pre-block the fixed/permeabilized cells with unlabeled XMG1.2 antibody (Cat. No. 554409) prior to staining. A suitable rat IgG1 isotype control for assessing the level of background staining on paraformaldehyde-fixed/saponin-permeabilized mouse or human cells is PE-R3-34 immunoglobulin (Cat. No. 557645); use at comparable concentrations to antibody of interest.